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Isolation, cloning, expression and mutation of Salvia fruiticosa and Salvia pomifera genes encoding terpene synthases and related enzymes

Two cDNA libraries, from Salvia fruticosa and Salvia pomifera were used as starting material for this research. An analysis of the expressed sequence tags (ESTs) was made in both cases and compared with ESTs from another member of the same Labiatae family (Origanum vugare), with a monocotyledon species (Oryza sativa), and with a dicotyledon with secondary growth (Populus sp). As expected, the gene distribution in the different categories suggested by the Munich Information Center for Protein Sequences (MIPS) was very similar for all the member of the Labiatae family. The analysis showed that sequences generally group together based on species relationships rather than function. This makes it difficult to use sequence information for the prediction of function. However, it could be noted that within closely related species, enzymes of similar function group together and in these cases, sequence information may be informative for predicting functionally important amino acids.

Identiferoai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:494154
Date January 2006
CreatorsIoannidis, Daphne
PublisherUniversity of Reading
Source SetsEthos UK
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation

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