In a series of experiments investigating a possible post-coitum contraceptive, it was observed that the numbers of corpora lutea (CL) of pregnant Sprague-Dawley (CD) rats were greater than in cyclic, unmated control females (p < 0.001). The ontogeny of these supplementary CL (SCL) was established by the recording the mean number of CL in pregnant Sprague-Dawley rats on days 1 to 9 post-coitum (day 1 being the first day post-coitum) using the gross phological dissection of the ovary. Compared to cyclic controls, an initial significant increase in mean CL numbers was recorded on day 1 post-coitum (p < 0.05) with a further significant increase between days 5 and 7 (p < 0.001). This phenomena was neither Sprague-Dawley strain, nor pregnancy specific, as a similar increase was also recorded for Wistar rats between days 4 and 7 postcoitum and identical increases in mean CL numbers were recorded in pseudopregnant rats of both strains. Histological studies of ovarian tissue confirmed the presence of newly formed CL on day 5 and 6 post-coitum and an absence of entrapped ova; follicular development was also present in early pregnancy with Graafian follicles evident on day 4 post-coitum. Plasma progesterone determinations revealed no increase in circulating progesterone as a result of SCL formation in either pregnant or pseudopregnant rats. Supplementary ovulations may be initiated by the preimplantation surge of oestrogen, on day 4 post-coitum, as treatment with the anti-oestrogen. Tamoxifen, inhibits their formation. Investigations into the presence of an appropriately-timed preovulatory surge of gonadotrophins in the early stages of pregnancy however, proved inconclusive. Supplementary ovulation occurs therefore between day 4 and 7 post-coitum in both pregnant and pseudopregnant Wistar and Sprague-Dawley rats, probably initiated by a consistent physiological event. This phenomena therefore obviously has an impact on the estimation of embryo mortality studies in this species.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:347864 |
| Date | January 1983 |
| Creators | Garside, Deborah A. |
| Publisher | London Metropolitan University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://repository.londonmet.ac.uk/3392/ |
Page generated in 0.0012 seconds