This thesis describes a number of discrete studies in which several novel peptides were discovered within the skin secretion of the African running frog, Kassina senegalens is, by use of reverse phase HPLC fractionation and mass spectrometry. The first of these was a prototype of a new class of antimicrobial hexadecapeptide with myotropic activity, named senegaii n, whose sequence was determined as: FLPFLlPALTSLISSL-amide (Chapter 4). In addition to the known antimicrobial peptides from this species, a novel eicosapeptide, (F lQHLlPLlPHAIQGIKDIF-amide), \vas also isolated and this peptide possessed growth inhibitory activity on cancer cell lines but was only found to be weakly haemolytic (Chapter 7). Two novel amidated peptides were isolated, both of which could induce muscle relaxation: WMSLGWSL-amide (WL-8 amide) (Chapter 5) and CYSAAKYPGFQEFlNRKYKSSRFamide (CF-23-KS) (Chapter 6), respectively. Also of significance. a Kunitz-type chymotrypsin inhibitor was isolated from the skin secretion and this represented the first of its type from an amphibian skin source (Chapter 3). Synthetic chemical replicates of all of these peptides, excepting the Kunitz inhibitor, were prepared in-house by employing solid-phase Fmoc chemistry.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:603482 |
| Date | January 2013 |
| Creators | Wang, Hui |
| Publisher | Queen's University Belfast |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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