The epidermal growth factor receptor (EGFR) and its family of ligands may be associated with the progression of atherosclerosis by mediating the proliferation and migration of endothelial cells and vascular smooth muscle cells. However there is some evidence that EGFR is expressed by cells ofthe monocytic lineage. This study was undertaken to investigate the expression of the EGFR on monocytes and macrophages and explore its potential function. The expression of the EGFR was studied using the monoclonal antibody ICR62.Flow cytometry revealed that the monocytic THP-l cell line expressed EGFR on its surface and specific EGFR reactivity was also observed by western blotting analysis. Whilst PMA differentiated THP-l cells failed to show cell surface expression of the receptor,immunocytochemical analysis revealed the receptor was localized to the nucleus. Human peripheral blood:-derived monocytes and monocyte-derived macrophages showed minimal cell surface expression of EGFR.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:505165 |
| Date | January 2008 |
| Creators | Dreux, Alys Catrin |
| Publisher | University of Surrey |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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