The aim of this project was to determine the best materials and surface treatments for soft tissue repair and to enhance our understanding of material / cell interactions by comparing the response of human cells growing on a selection of currently approved and novel biomaterials. This study focused on comparing the materials and also investigated the effect of modifying the surfaces using gas plasma and other treatments with the aim of enhancing cell growth. In addition, chitosan was studied to examine the reported bacteriostatic effect and promotion of human cell growth. Chitosan has many properties but this research focused on its reported acceleration of wound healing haemostatic and bacteriostatic properties. To examine the bacteriostatic properties of chitosan, a number of experimental designs were used. The bacteriostatic study led onto a selection of means to incorporate chitosan into/onto some of the biomaterials being tested. A selection of biomaterials were examined for their ability to support tissue growth in native and surface modified forms (plasma treatment/ chitosan treatment). Cells were seeded on the samples and the growth of the cells was measured at weekly intervals. The outcome of this research was that the optimal material for soft tissue repair was found to be polyurethane with an ammonia plasma treatment. This can be made into a mesh prosthesis for hernia repair and can be coated with chitosan to inhibit bacterial colonisation if required.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:527384 |
| Date | January 2010 |
| Creators | Nairn, Michael Douglas |
| Publisher | Heriot-Watt University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://hdl.handle.net/10399/2330 |
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