The aim of this project was to investigate into the effect of the Loa mutation in the function of the cytoplasmic dynein complex. Protein-protein interaction assays were performed on NIH3T3, NSC34 and HEK293T cells transfected with a 2 kb fragment of the wild type or mutant dynein heavy chain gene 1 bearing the Loa mutation and seven different intermediate chain isoforms. The results indicated a 2.5 fold increase in the binding affinity of the mutant dynein heavy chain 1 727 amino acid fragment for the dynein intermediate chain gene 2 isoform A, compared to the wild type.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:494921 |
| Date | January 2009 |
| Creators | Soura, Violetta |
| Publisher | University of Sussex |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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