The human plasma proteome is potentially rich in novel biomarkers for the diagnosis, prognosis of disease and drug development. In the quest for low abundant biomarkers, the problems associated with the complexity of sample and vast dynamic range in concentration have to be overcome. Such strategies include the depletion of the most abundant proteins from plasma, the employment of prefractionation techniques and multidimensional separations. Mass spectrometry can be used to identify and quantify these biomarkers. The most abundant proteins can be depleted from plasma using antibody or dye based affinity columns. Several abundant protein depletion kits were investigated with the aim of selecting the most effective kit for subsequent analyses.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:500312 |
| Date | January 2008 |
| Creators | Burn, Ross Thomas |
| Publisher | Queen Mary, University of London |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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