The precise mechanisms by which human intervertebral discs (IVD) respond to load remains unknown and are a challenge to investigate in vitro. In this study, a novel mechanical loading system was devised, built, tested and successfully used to apply hydrostatic pressure to nucleus pulposus (NP) and annulus fibrosis (AF) cells and explants obtained from human nondegenerate and degenerate IVD. Cells were removed from the IVD, cultured in monolayer to generate sufficient numbers and subsequently embedded in alginate constructs prior to application of dynamic cyclic hydrostatic pressure at physiological levels.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:493463 |
| Date | January 2007 |
| Creators | Frain, Jennie |
| Publisher | University of Manchester |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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