The analysis of trace amounts of DNA has become one of the most powerful tools in forensic science. DNA profiles can be obtained from as little as 100 pg of purified DNA derived from skin cells. These skin cells may have been transferred to objects by direct contact such as handling or wearing clothing. These are examples of primary transfer but other types of transfer are known to occur. In this work, the primary transfer of skin cells to a variety of substrates is illustrated and a new method of generating a DNA profile proposed. The generation of numerous DNA profiles directly from fabrics and other substrates confirmed the existence of both cellular transfer and the transfer of free DNA. DNA typing methods from various substrates of free DNA, which omits the requirement for prior DNA extraction, were developed. Differences in the resulting DNA profiles from a range of natural and artificial fabrics are illustrated.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:502278 |
| Date | January 2009 |
| Creators | Pekarek, Vera |
| Publisher | University of Strathclyde |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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