This thesis has focussed on the development and application of multidimensional liquid phase protein separations for proteomic analysis. Multidimensional liquid phase chromatography was developed utilising ion exchange chromatography using both cation and anion exchange colums in series in the first dimension. Alternatively, liquid isoelectric focusing protein fractionation was developed first dimension protein separation. Reversed phase liquid chromatography was eloped as a second dimension system, utilising 500 |Lim i.d capillary monoliths. Subsequently, the proteins collected from second dimension were in-solution digested using trypsin and analysed by mass spectrometry. In addition, reversed ase capillary chromatography was coupled directly to ESI-MS to determine intact molecular weights of proteins in a combined top down, bottom up proteomic approach.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:505476 |
| Date | January 2009 |
| Creators | Assiddiq, Bobby Fachrizal |
| Publisher | University of Sheffield |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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