The requirement for complex, therapeutic proteins that possess the correct post-translational modifications has resulted in mammalian cells, in particular Chinese hamster ovary (CHO) cells, being widely used in die biopharmaceutical industry, In the creation of mammalian cell lines plasmid DNA carrying the gene of interest integrates randomly into die host cell genome. Integration into different chromatin domains results in variable levels of gene expression between cell lines due to gene silencing mechanisms. In addition, cell lines often show unstable protein production during long-term culture. Variable and unstable recombinant protein expression means that a large number of clones need to be screened in order Therefore there is a necessity to overcome these gene silencing mechanisms, with the intention to accelerate process development of recombinant protein production in mammalian systems.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:493616 |
| Date | January 2008 |
| Creators | Croxford, Alexandra Sarah |
| Publisher | University of Manchester |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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