Prototypic foamy virus (PFV)-based vectors are currently made by transient transfection. Continuous vector production by cells in which PFV proteins are stably led would allow rapid, reproducible generation of large quantities of vector. Previous attempts at constructing packaging cell lines have resulted in very low titre production. Here, we utilise a method described to generate a stable HIV-1 producing cell-line in an attempt to produce high titres of PFV vector. PFV gag-pol or env genes were stably transduced into a variety of cell lines using an MLV-based delivery system. The resultant cell lines were positive for stable DNA integration and for high levels of protein expression, and produced between 10³ and 10⁵ infectious units of PFV vector. However the cells gradually reduced protein production which finally ceased completely after 4-5 weeks, possibly due to cytotoxicity.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:506165 |
| Date | January 2009 |
| Creators | Menon, Dev Christophe |
| Publisher | Imperial College London |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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