Cryptococcus neofomans causes up to 80% of cryptococcal meningitis cases in HIV positive individuals, resulting in significant morbidity and mortality within this patient group. Heat-shock proteins (Hsps) are a group of proteins play vital functions in the folding and unfolding or translocation of proteins, as well as in the assembly and disassembly of protein complexes. Many heat-shock proteins have been identified in fungi, such as Hsp70 in C. neoformans and Hsp90 in C. albicans. Mycograb® is a recombinant antibody derived against the LKVIRKNIV epitope of candidal Hsp90. In the present thesis, anti-Hsp90 polyclonal antibody was used in the immunoelectron microscopy and immunoblot assays to demonstrate the cellular localization and induction of Hsp90 in C. neoformns under various stress factors.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:493948 |
| Date | January 2009 |
| Creators | Alakeel, Raid Abdulrahman |
| Publisher | University of Manchester |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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