The broad aim of this work was to investigate and optimise the properties of calcium phosphate bone cements (CPCs) for use in vertebroplasty to achieve effective primary fixation of spinal burst fractures. An in vitro sample production method was developed and the effect of CPCs on the pH and Ca concentration of culture medium assessed. The effect of collagen incorporation into CPCs was investigated in vitro, necessitating sterilisation of the samples. y irradiation was found to be the most effective technique. In vitro analysis of two CPCs found no significant difference in their biological properties, although their ability to promote differentiation was significantly higher than that seen in a PMMA control. Analysis of collagen augmented CPCs found that the incorporation of 1 wt% collagen resulted in slightly higher, though not clinically relevant, level of cytotoxicity. Despite this, cells were shown to proliferate equally well on each of the formulations. Incorporation of marine collagen did not significantly affect the level of differentiation observed; however, this level was significantly decreased when bovine collagen was considered. The CPCs investigated were better tolerated in vivo than PMMA. Significantly more bone apposition was observed on the surface of CPCs compared to PMMA and a small amount of remodelling was seen on the CPCs.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:602791 |
| Date | January 2013 |
| Creators | Palmer, Gareth Iwan |
| Publisher | Queen's University Belfast |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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