Human islet transplantation would offer a less invasive and more physiological alternative than whole pancreas transplantation and insulin injections respectively for the treatment of diabetes mellitus if islet graft survival can be improved, initial recipient post-transplant insulin independence declines to <10% after 5 years. Factors contributing to graft failure include enzymatic disruption of the islet microenvironment during isolation, diabetogenic effects of immunosuppressants and metabolic stress resulting from slow revascularisation. Aims: To investigate the effect of co-culture in both static (SC) and rotational culture (RC) of BRINBDU beta-cells (Dl 1) and human umbilical vein endothelial cells (HUVEC) on D11 insulin secretion; and the effect of a thiazolidmedione (TZD) on Dl 1 function and HUVEC proliferation. To assess the effect of culture media, SC, RC and a TZD on human islet morphology, insulin secretion and VEGF production. To initiate in vivo protocol development for assessment of revascularisation of human islet grafts.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:497373 |
| Date | January 2008 |
| Creators | Paget, Michelle B. |
| Publisher | Aston University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://publications.aston.ac.uk/15366/ |
Page generated in 0.0014 seconds