Since their introduction to dentistry, calcium silicate based cements have been mainly used for endodontic applications, principally due to their long setting time. Recently, a new formulation of this cement was produced as a coronal restorative material. The aim of this project was to study the nature and dynamics of the interface between this calcium silicate based dental restorative material (BiodentineTM) with human dentine in comparison with glass ionomer cement, and to investigate its capability to induce dentine remineralisation. Different optical, microscopic, and fluorescent labelling techniques have been applied; such as tandem scanning and laser scanning confocal microscopy, which were both used with cement labelling and micropermeability tests to evaluate the interfacial morphology and microscopic appearance along with scanning electron microscopy. Additionally, two-photon fluorescence microscopy was applied in conjugation with Tetracycline labelling to study dentine remineralisation induced by the Biodentine cement; this novel combination provided a useful technique for the observation of mineral formation inside the organic matrix of demineralised dentine when aged in an in-vitro model. Fluorescence lifetime imaging and second harmonic generation imaging were also used for the characterisation of re-mineralisaion and collagen denaturation respectively. For the chemical analysis, Raman spectroscopy was applied to analyse the chemical composition of the cement and the changes associated with its hydration and ageing in different conditions. Micro-Raman imaging was also applied to quantify and model the infiltration of the cement’s hydration products into the dentine. Results indicated an interactive interface between the Biodentine and sound dentine, mediated by the alkaline caustic effect of the cement on the dentine’s organic component, which was associated with mineral transfer, and led to the formation of what we described as a “Mineral Infiltration zone” (MIZ). This later explained the ability of Biodentine to induce remineralisation of dentine and the formation of apatite structures, which indicated the bioactivity of the cement.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:628365 |
| Date | January 2013 |
| Creators | Atmeh, Amre |
| Publisher | King's College London (University of London) |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | https://kclpure.kcl.ac.uk/portal/en/theses/optical-characterisation-of-the-interaction-between-calciumsilicate-based-dental-restorative-materials-and-dentine(8949ef19-09fc-4303-87be-c6a3835d21ea).html |
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