Expression vectors based on the bipartite Cowpea Mosaic Virus (CPMV) have been investigated for use in molecular farming in plants. Agroinfiltration into Nicotiana benthamiana of two full-length RNA-2 constructs containing different marker genes in the presence of RNA-1 showed that two foreign proteins can be efficiently expressed within the same cell in inoculated tissue. However, segregation of the RNA-2 molecules was observed upon systemic spread of the recombinant viruses. Thus efficient assembly of heteromeric proteins is likely to occur only in inoculated tissue. Therefore vectors based on full-length RNA-2 molecules do not have an advantage over vectors based on deleted versions of RNA-2 that are restricted to infiltrated tissue.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:502194 |
| Date | January 2008 |
| Creators | Sainsbury, Frank |
| Publisher | University of East Anglia |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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