A metabolomics platform technology was used to study known traits of Thai rice, brown planthopper (BPH) resistance trait. Three rice varieties with different BPH resistance capacity, namely BPH-susceptible KD cultivar, BPH-resistant IL7 containing Bph 3 and BPH-resistant+ IL308 containing Bph 3 and terpene synthase (TPS) genes, were analyzed to identify differential metabolomics profiles between them, with and without BPH infestation at different time points (Days 1, 2, 3, 4 and 8). Metabolic profiles were obtained using the analytical methods including Proton Nuclear Magnetic Resonance (1 H NMR) Spectroscopy, Gas Chromatography-Mass Spectrometry (GC-MS) and Ultra Performance Liquid Chromatography Quadrupole Time of Flight -Mass Spectrometry (UPLC-QToF-MS). The metabolomics data were analysed using multivariate statistical analysis to reveal metabolite markers underlying those traits. Different physiological responses from the three varieties were observed in leaves which were dependent on the level of BPH resistance. Untargeted metabolome profiling of rice leaves obtained by 1 H NMR provided thirty primary metabolites profiles revealing the separation between early and late responses. UPLC-QToF-MS method provided more sensitivity and coverage of compounds, hence, it revealed the effective secondary metabolites differences in the early response between the resistant+ IL308 and the other examined (KD and IL7) varieties. Besides, target fatty acid analysis identified fourteen potential fatty acid associated with the BPH resistance. The overall metabolic pathways obtained by the three different methods suggested that BPH infestation causes the metabolic perturbations in transamination, amino acid metabolism, shikimate, purine/pyrimidine, gluconeogenesis, phenylpropanoid and fatty acid pathways. The susceptible and the resistant rice varieties were likely to employ different pathways to fight against BPH infestation. This study identified several potential metabolic pathways of Thai Jasmine rice and its BPH-isogenic lines that can play a role in acclimatization and defense mechanisms against BPH infestation.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:696327 |
| Date | January 2015 |
| Creators | Uawisetwathana, Umaporn |
| Publisher | Queen's University Belfast |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
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