This thesis explores the use of polymer nanoreactors in catalysis as well as the use of water-soluble polymers in enzyme stabilisation. Chapter 1: An introduction to the literature currently covering the research topics relevant to the techniques used within this thesis, providing further background on the fundamental enzyme theory that is to be explored Chapter 2: This looks at the techniques available for tethering the catalyst triazabicyclodec-5-ene to the hydrophobic block of an amphiphilic block copolymer, synthesised by nitroxide mediated polymerisation and the assembly of these polymers. Chapter 3: The previously synthesised nanoreactors were used to catalyse Michael additions with varied loadings within the core of the micelles. Chapter 4: Looks into the effect of using water soluble polymers of varying molecule weights on the activity of Lipases Circular dichroism spectroscopy and stain tests were used as tools to indicate changes to the native structure and activity. Chapter 5: Thermostability of lipase was investigated, and the use of the water-soluble polymers to extend the lifetime of the enzyme in solution was investigated through monitoring changes in the melting temperatures as well as stability studies.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:685183 |
| Date | January 2016 |
| Creators | Farrance, Katherine |
| Publisher | University of Warwick |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://wrap.warwick.ac.uk/78797/ |
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