The human ABF-1 gene is expressed in activated B-cells and Epstein-Barr virus immortalized lymphoblastoid cell lines. ABF-1 represents the only member belonging to the basic helix-loop-helix (bHLH) family of transcription factors whose expression pattern is restricted to B cells. ABF-1 forms heterodirner complexes with E2A to modulate gene transcription. Here we report the isolation often novel ABF-1 splice products from a human B-cell plasmacytoma cell line. Analysis of the nucleotide sequence revealed approximately five clones harboring changes within the coding region of the reported full-length ABF-1 eDNA. In some cases, these changes resulted in the elimination of the coding region specifying the bHLH domain and likely affect DNAbinding and dirnerization of ABF-1. For the other retrieved splice products, the changes within the eDNA were localized to the 3' untranslated region (UTR), which likely indicate the potential for translational regulation of the ABF -1 gene.
| Identifer | oai:union.ndltd.org:pacific.edu/oai:scholarlycommons.pacific.edu:uop_etds-1547 |
| Date | 01 January 2000 |
| Creators | Mora, Noe |
| Publisher | Scholarly Commons |
| Source Sets | University of the Pacific |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | University of the Pacific Theses and Dissertations |
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