Lam Kit. / Thesis submitted in: July 2002. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 141-153). / Abstracts in English and Chinese. / Acknowledgment --- p.I / Table of Content --- p.II / List of Tables --- p.VII / List of Figures --- p.IX / Abbreviations --- p.XIV / Abstract --- p.XVI / 論文摘要 --- p.XIX / Chapter CHAPTER 1 --- INTRODUCTION --- p.1 / Chapter 1.1 --- Hepatitis B --- p.1 / Chapter 1.1.1 --- Brief Introduction of HBV --- p.1 / Chapter 1.1.2 --- History of Hepatitis B Virus --- p.2 / Chapter 1.1.3 --- Hepatitis B Virus Infection around the World --- p.4 / Chapter 1.1.4 --- Hepatitis B Virus Infection in Hong Kong --- p.5 / Chapter 1.1.5 --- Hepatitis B Virus Infection in China --- p.7 / Chapter 1.1.5.1 --- Update of HBV Infection in China --- p.7 / Chapter 1.1.5.2 --- Problems in China --- p.7 / Chapter 1.2 --- Hepatitis B Virology --- p.8 / Chapter 1.2.1 --- Hepadnaviridae Family --- p.8 / Chapter 1.2.2 --- HBV Particles Types --- p.9 / Chapter 1.2.3 --- The HBV Genome --- p.10 / Chapter 1.2.4 --- The Life Cycle of HBV --- p.12 / Chapter 1.2.5 --- Hepatitis B Surface Antigen (HBsAg) --- p.17 / Chapter 1.3 --- HBV Transmission --- p.19 / Chapter 1.4 --- HBV Therapy --- p.19 / Chapter 1.5 --- Phyllanthus Species --- p.22 / Chapter 1.6 --- Alexander Cells --- p.26 / Chapter 1.7 --- Objectives --- p.29 / Chapter CHAPTER 2 --- COMPARISONS OF AQUEOUS AND ORGANIC EXTRACTS OF THREE PHYLLANTHUS SPECIES OF THEIR IN VITRO ANTI-HBV EFFECTS --- p.30 / Chapter 2.1 --- Introduction --- p.30 / Chapter 2.2 --- Materials and Methods --- p.30 / Chapter 2.2.1 --- Materials --- p.30 / Chapter 2.2.1.1 --- Phyllanthus species --- p.30 / Chapter 2.2.1.2 --- "Chemicals, Antibodies and Instrument" --- p.31 / Chapter 2.2.2 --- Extraction Methods --- p.32 / Chapter 2.2.2.1 --- Aqueous Extraction --- p.33 / Chapter 2.2.2.2 --- Organic Extraction --- p.33 / Chapter 2.2.3 --- Cell line --- p.33 / Chapter 2.2.4 --- Toxicity of Extracts --- p.34 / Chapter 2.2.5 --- IMx Assay --- p.34 / Chapter 2.2.6 --- Semi-quantitative RT-PCR --- p.35 / Chapter 2.2.6.1 --- RNA Extraction --- p.35 / Chapter 2.2.6.2 --- RT-PCR --- p.36 / Chapter 2.2.7 --- Western Blotting --- p.37 / Chapter 2.2.7.1 --- Preparation of Protein Samples --- p.37 / Chapter 2.2.7.2 --- Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) --- p.37 / Chapter 2.2.7.3 --- Protein Transfer --- p.38 / Chapter 2.2.7.4 --- Immumnoblotting --- p.39 / Chapter 2.2.7.5 --- Protein Assay --- p.39 / Chapter 2.3 --- Results --- p.40 / Chapter 2.3.1 --- Toxicity of the Extracts --- p.40 / Chapter 2.3.2 --- Effects on HBsAg Secretion and Viral Gene Expression --- p.45 / Chapter 2.3.2 --- Analysis of Intracellular Viral Proteins --- p.58 / Chapter 2.4 --- Discussion --- p.63 / Chapter CHAPTER 3 --- ISOLATION AND CHARACTERIZATION OF ACTIVE COMPONIENT FROM AN ORGANIC EXTRACT OF PHYLLANTHUS URINARIA (GUANGDONG) --- p.68 / Chapter 3.1 --- Introduction --- p.68 / Chapter 3.2 --- Materials and Methods --- p.69 / Chapter 3.2.1 --- Materials --- p.69 / Chapter 3.2.2 --- Methods --- p.70 / Chapter 3.2.2.1 --- Ethanol Extraction --- p.70 / Chapter 3.2.2.2 --- Partitions --- p.70 / Chapter 3.2.2.3 --- Column Purification --- p.71 / Chapter 3.2.2.4 --- Analytical Thin Layer Chromatographic (TLC) --- p.71 / Chapter 3.2.2.5 --- Crystallization --- p.71 / Chapter 3.3 --- Results --- p.72 / Chapter 3.3.1 --- Analysis of Four Fractions after Partitions --- p.72 / Chapter 3.3.2 --- Screening of the Active Fraction after Column Chromatography of Fraction B --- p.76 / Chapter 3.3.3 --- Screening of the Active Fraction after Column Chromatography of Fraction6 --- p.79 / Chapter 3.3.4 --- Crystallization and Identification of the Isolated component --- p.82 / Chapter 3.3.5 --- Study Anti-HBV effects of pheophorbide a --- p.91 / Chapter 3.4 --- Discussion --- p.97 / Chapter CHAPTER 4 --- STUDY OF PRE S I PROMOTER ACTIVITY OF HBV --- p.103 / Chapter 4.1 --- Introduction --- p.103 / Chapter 4.2 --- Materials and Methods --- p.108 / Chapter 4.2.1 --- Materials --- p.108 / Chapter 4.2.2 --- Methods --- p.109 / Chapter 4.2.2.1 --- Cell line --- p.109 / Chapter 4.2.2.2 --- Clonning of Pre SI Promoter from HBV Genome --- p.109 / Chapter 4.2.2.3 --- Gene Clean --- p.110 / Chapter 4.2.2.4 --- Restriction Enzyme Digestion --- p.111 / Chapter 4.2.2.5 --- Synthesis of T-Overhang EcoR V Cut pBluescript® II KS (-) --- p.111 / Chapter 4.2.2.6 --- Ligation --- p.112 / Chapter 4.2.2.7 --- DH5α Competent Cells Preparation --- p.112 / Chapter 4.2.2.8 --- Transformation --- p.113 / Chapter 4.2.2.9 --- Plasmid Purification --- p.113 / Chapter 4.2.2.10 --- Transfection --- p.114 / Chapter 4.2.2.11 --- Luciferase Assay --- p.115 / Chapter 4.3 --- Results --- p.116 / Chapter 4.3.1 --- Cloning of the Pre S I Promoter --- p.116 / Chapter 4.3.2 --- Sequences of the Pre S I Promoter --- p.121 / Chapter 4.3.3 --- Pre S I Promoter Activities in Hep 3B Cell Line --- p.123 / Chapter 4.3.4 --- Effects of Herbal Extracts on Pre S I Promoter --- p.126 / Chapter 4.4 --- Discussion --- p.130 / Chapter CHAPTER 5 --- GENERAL DISCUSSION --- p.134 / REFERENCES --- p.141
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324699 |
| Date | January 2004 |
| Contributors | Lam, Kit., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xx, 153 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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