acase@tulane.edu / Osteoporosis is a prevalent bone metabolic disease characterized by bone
fragility. As a key pathophysiological mechanism, the disease is caused by excessive
bone resorption (by osteoclasts) over bone formation (by osteoblasts). Peripheral blood
monocytes (PBMs) represent a major systemic cell type for bone metabolism by serving
as progenitors of osteoclasts and producing cytokines important for osteoclastogenesis.
Our lab previously used microarray-based transcriptomics profiling to identify a list of
novel genes for osteoporosis.
My work is to further investigate the factors and regulatory network in
osteoporosis, using microarray data of monocytes from subjects with extremely high/low
hip bone mineral density. 1) We performed a pathway analysis and developed a novel
approach to correct the “crosstalk” phenomenon which is caused by overlapping genes.
2) We analyzed the long non-coding RNA (lncRNA) profile by re-annotating exon array
and predicted the regulatory mechanism of lncRNAs on protein coding genes in bone
metabolism. 3) We identified the important potential transcription factors for
osteoporosis and inferred the regulatory mechanism which exists between transcription
factors and target genes in bone metabolism.
My findings not only reported the key regulatory factors (lncRNAs and
transcriptional factors) contributes to bone metabolism, but also explored the potential
regulatory networks in osteoporosis. / 1 / Yu Zhou
| Identifer | oai:union.ndltd.org:TULANE/oai:http://digitallibrary.tulane.edu/:tulane_79048 |
| Date | January 2018 |
| Contributors | Zhou, Yu (author), Deng, Hong-Wen (Thesis advisor), School of Science & Engineering Cell and Molecular Biology (Degree granting institution) |
| Publisher | Tulane University |
| Source Sets | Tulane University |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | electronic, 115 |
| Rights | No embargo, Copyright is in accordance with U.S. Copyright law. |
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