Chlorophyll fluorescence imaging has now become a versatile and standard tool in fundamental and applied plant research. This method captures time series images of the chlorophyll fluorescence emission of whole leaves or plants upon various illuminations, typically combination of actinic light and saturating flashes. Several conventional chlorophyll fluorescence parameters have been recognized that have physiological interpretation and are useful for, e.g., assessment of plant health status and early detection of biotic and abiotic stresses. Chlorophyll florescence imaging enabled us to probe the performance of plants by visualizing physiologically relevant fluorescence parameters reporting physiology and biochemistry of the plant leaves. Sometimes there is a need to find the most contrasting fluorescence features/parameters in order to quantify stress response at very early stage of the stress treatment. The conventional fluorescence utilizes well defined single image such as F0, Fp, Fm, Fs or arithmetic combinations of basic images such as Fv/Fm, PSII, NPQ, qP. Therefore, although conventional fluorescence parameters have physiological interpretation, they may not be representing highly contrasting image sets. In order to find the effect of stress treatments at very early stage, advanced statistical techniques, based on classifiers and feature selection methods, have been developed to select highly contrasting chlorophyll fluorescence images out of hundreds of captured images. We combined sets of highly performing images resulting in images with very high contrast, the so called combinatorial imaging. The application of advanced statistical methods on chlorophyll fluorescence imaging data allows us to succeed in tasks, where conventional approaches do not work. This thesis aims to explore the application of conventional chlorophyll fluorescence parameters as well as advanced statistical techniques of classifiers and feature selection methods for high-throughput screening. We demonstrate the applicability of the technique in discriminating three species of the same family Lamiaceae at a very early stage of their growth. Further, we show that chlorophyll fluorescence imaging can be used for measuring cold and drought tolerance of Arabidopsis thaliana and tomato plants, respectively, in a simulated high ? throughput screening.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:55608 |
| Date | January 2012 |
| Creators | MISHRA, Anamika |
| Source Sets | Czech ETDs |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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