This diploma thesis is concerned about the PCR-RFLP method for identification of yeasts genus Saccharomyces from leaves, grapes and must of white wine. The thesis describes an obtaining of pure cultures of yeasts, isolation DNA and amplification of specific segments of DNA using PCR method. Splitting of this segments by restriction endonukleases and a detection of final fragments by zone electrophoresis are mentioned too. Length of fragments and numbers of restriction fragments, which are characteristic of every species, enable a determination of microorganisms and their inclusion into the system. The basic informations on yeasts and molecular methods for their identification are named in the theoretical part of the thesis. The main part of the thesis deals with the processing of grapes of grape-wine and the manufacturing of white wine.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216401 |
| Date | January 2008 |
| Creators | Škodová, Anna |
| Contributors | Štros, Michal, Vránová, Dana |
| Publisher | Vysoké učení technické v Brně. Fakulta chemická |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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