ADP Ribosylation Factor 1 (ARF1) is a universal small GTP binding protein
which has an important role in vesicular trafficking between endoplasmic
reticulum and Golgi. ARF1 is a basic component of Coat Protein I (COPI) vesicles
which have functions in both formation of coatomer complex and recruitment of
cargo proteins. In this study, the expression ARF1 was analyzed in pea (P. sativum
L. cv. Araka) grown at different developmental stages. Because of the differential
hormonal levels at corresponding stages, the effects of hormones on ARF1
expression were also studied.
The results of present research show that ARF1 expression in embryos and 2 days
grown plants after germination is lower when compared to 6 days grown plants. In
order to see the hormonal effect, 3 weeks old plants were supplied with 50µ / M of
each hormone for 3 times on alternate days. Protein extraction, cell fractionation,Western blot was carried out and immunoblot analysis was conducted with
AtARF1 polyclonal antibodies.
It was shown that, in pea shoots, abscisic acid and gibberellin increases the
inactive GDP bound ARF1 by hydrolyzing ARF-GTP through activating ARFGTPase
activating protein (ARF-GAP) or partially inhibiting ARF-Guanine
Nucleotide Exchange Factor (ARF-GEF). In roots, ARF-GDP (cytosolic fraction),
ARF-GTP (microsomal fraction) and total amount of ARF1 (13.000 x g
supernatant fraction) were down regulated by ~11, ~19 and ~11 fold respectively
with the application of gibberellin / and by ~11, ~7 and ~3 fold respectively with
the application of abscisic acid / when compared to control plants. These results
indicate the importance of plant hormones in the regulation of ARF1 in pea.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12608902/index.pdf |
| Date | 01 September 2007 |
| Creators | Ertekin, Ozlem |
| Contributors | Yucel, Meral |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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