A process-control temperature system was designed and developed for facilitating somatic cell research on eastern oyster Crassostrea virginica. The system consisted of sixteen independent tanks capable of individual temperature regimes controlled by a central computer. Food grade 250-L tanks were used to hold 60 oysters per tank. Each tank was equipped with heating and cooling mechanisms. Immersion heaters were used for heating and a pump and a stainless steel heat exchangers immersed in chilled water (~ 7°C) were used for cooling. Type-T thermocouples along with a CIOEXP32 multiplexer board (Measurement Computing Inc.) were used to measure water temperatures. Control was achieved via a pair of analog and digital (A/D) boards that actuated heaters and pumps through solid state relays.
The performance of the system was evaluated in two experiments for studies on somatic cell proliferation in eastern oysters. In the first experiment, the system was programmed to maintain constant temperatures of 10, 15, and 20°C for a 20-day period. The system was able to maintain temperatures within ± 0.2°C. In the second experiment, diurnal fluctuations of 20 ± 1°C, 20 ± 5°C, 15 ± 1°C, 15 ± 5°C, and 15 ± 10°C were imposed. The system was able to simulate water temperatures within ± 0.2°C for regimes 20 ± 1°C, 20 ± 5°C, 15 ± 1°C.
A computer program Realwire for measuring and simulating real-time water temperatures was developed using freely available public domain software called Python and GNUwget. The software was able to measure and simulate real-time water temperatures.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-07072004-114606 |
| Date | 08 July 2004 |
| Creators | Kolar, Praveen |
| Contributors | Steven G. Hall, Terrence R. Tiersch, Jerome F. La Peyre, Charles J. Monlezun |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-07072004-114606/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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