The published benefits of polymeric nanoparticles as a system for antioxidant delivery have encompassed topics of improved oral delivery, bioavailability, and modified release to name a few. Generally accepted as safe polymers Poly(lactic-coglycolic acid) and the mucoadhesive chitosan were selected to entrap alpha-tocopherol
and lutein. This study proposed to assess the physical stability of PLGA and Chi/PLGA nanoparticles with entrapped bioactives in varying biological suspension medias ( water,
pbs, hbss and cell media). Also, to assess the chemical stability of the entrapped
antioxidants lutein and alpha-tocopherol within the same media as deemed relevant for
in-vitro and and in-vivo studies. Of the tested media, physical stability was not media
dependent for the PLGA(AO) or PLGA/Chi (AO) particles. Neither the size nor the PDI
appeared significantly affected by the media it was re-suspended. Entrapped alphatocopherol
remained chemically stable throughout the time suspended in the various biological mediums. While the lutein emulsion stability decreased over time, the NP(L)demonstrated improved stability. Also, the entrapped lutein stability was not dependent
on the selected medium. Identifying any potential effects of suspension media on polymeric nanoparticles and the entrapped bioactive affords the opportunity to improve analysis of more robust or complex nanoparticle studies. Also, presented are considerations that should be taken prior to design of an experiment.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-07132014-234534 |
| Date | 27 July 2014 |
| Creators | Whaley, Meocha |
| Contributors | Devireddy, Ramachandra, Moldovan, Dorel, Sabliov, Cristina |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-07132014-234534/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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