The prevalence of antimicrobial resistance (AMR) phenotypes and genotypic
characteristics (Class 1 integron and AMR gene cassettes) in commensal Escherichia coli
(EC) and vancomycin resistant Enterococcus faecium (EF) isolated from humans and
swine in a semi-closed, integrated farrow-to-fork population were evaluated in a crosssectional
study. Our objective was to establish baseline antimicrobial resistance patterns
and to evaluate the stability of isolate recovery phenotype within multiple grab samples per
collection day and over multiple biweekly samples collected during a period of several
months. This data will serve as a baseline for continuing longitudinal studies within the
population. These continuing studies should produce the first comprehensive
epidemiological data to document the transmission dynamics of antimicrobial resistance in
the farrow-to-fork continuum. Outcome variables assessed included: phenotypic resistance
in EC, pan-susceptibility, multi-resistance and genotypic resistance. Potential predictor
variables included: 1) host species, 2) unit, 3) unit type, 4) housing cohort by species, and
5) time of day. There were significant differences (p<0.05) between host species with
swine at higher odds for both single and multiple resistance. There were also differences
in resistance based on unit location, unit-type, and housing cohort within both humans and
swine. Our study found no significant differences (p>0.05) in resistance between swine
workers and non-swine workers with the sole exception of resistance to cephalothin, with
non-swine workers at 1.89 higher odds for resistance (p=0.02). A total of 17 VRE were
isolated from human wastewater samples, and to the authorÂs knowledge these represent
the first environmentally isolated VRE in the U.S. Several unique multi-resistance
phenotypes were observed and future evaluation of AMR phenotype in continuing
longitudinal studies provides a unique opportunity to study phenotypic patterns and
dissemination through the study population.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/3146 |
| Date | 12 April 2006 |
| Creators | Campbell, Linda Diane |
| Contributors | Scott, H.M. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Thesis, text |
| Format | 390705 bytes, electronic, application/pdf, born digital |
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