Plant biomass serves as a carbon and energy source for Streptomyces spp. which
secrete degradative enzymes capable of breaking down the complex plant biomass into
simple saccharides. Hemicellulose is a major component of plants and is composed of
five and six carbon sugars, such as xylose and glucose. Enzymatic degradation of
hemicellulose to obtain desired sugars has been a cornerstone of many industries, as well
as the subject of worldwide research for additional sources of efficient enzymes for
substrate conversion. In this study, environmentally-derived Streptomyces isolates were
screened for their ability to hydrolyze oat-spelt and birchwood xylan in agar-based high
throughput activity screens. Of the isolates tested, eight displayed high levels of
substrate-degrading activity and were chosen for further characterization which included
16S rRNA gene analysis, microscopic analysis from both liquid and agar grown cultures,
xylanase-specific activity, lignin peroxidase production and indole acetic acid
production.Qualitative assessment of extracellular lactone signalling for all eight isolates
was also performed. Putative lactone signalling was observed for Streptomyces isolates
JLS1-C4, JLS1-A6, JLS2-D6 and KT1-B1 which exhibited xylanase-specific activities of
0.622 μmol/min/mg, 0.0243 μmol/min/mg, 0.721 μmol/min/mg, and 0.706 μmol/min/mg
respectively. Streptomyces isolates JLS1-F12 and JLS1-C12 did not exhibit lactone
signalling but did exhibit xylanase-specific activities of 0.125 μmol/min/mg and 0.0688
μmol/min/mg respectively. No xylanase-specific activity was detected for isolates JLS2-
C7 and KT1-B8; however lactone signalling was observed for isolate KT1-B8.
Streptomyces isolate JLS1-A6 degraded birchwood xylan optimally at pH 4 and 28°C
with a maximal xylanase activity of 1.56 x10-3 μmol/min/mg. / UOIT
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OOSHDU.10155/262 |
| Date | 01 July 2012 |
| Creators | Thompson, Khalil |
| Contributors | Strap, Janice |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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