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Differential regulation of gonadotropin expression in the goldfish, Carassius auratus, by hypothalamic dopamine and pituitary activin.

Yuen Chi Wai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 84-106). / Abstracts in English and Chinese. / Abstract (in English) --- p.ii / Abstract (in Chinese) --- p.iv / Acknowledgement --- p.vi / Table of Contents --- p.vii / List of Figures --- p.xii / Symbols and Abbreviations --- p.xv / Scientific names --- p.xvii / Chapter Chapter 1 --- General Introduction --- p.1 / Chapter 1.1 --- Pituitary --- p.1 / Chapter 1.2 --- Gonadotropins (GTHs) --- p.3 / Chapter 1.2.1 --- Structure --- p.3 / Chapter 1.2.2 --- Function --- p.5 / Chapter 1.2.3 --- Regulation --- p.7 / Chapter 1.2.3.1 --- Neuroendocrine hypothalamic regulators --- p.9 / Chapter 1.2.3.1.1 --- Gonadotropin-releasing hormone (GnRH) --- p.9 / Chapter 1.2.3.1.2 --- Dopamine (DA) --- p.11 / Chapter 1.2.3.2 --- Endocrine regulators from the gonads --- p.12 / Chapter 1.2.3.2.1 --- Gonadal steroids (T and E2) --- p.12 / Chapter 1.2.3.2.2 --- Negative steroid effect on pituitary GTH regulation --- p.12 / Chapter 1.2.3.2.3 --- Positive steroid effect on pituitary GTH regulation --- p.13 / Chapter 1.2.3.3 --- Paracrine regulators from within the pituitary --- p.14 / Chapter 1.3 --- Activin --- p.14 / Chapter 1.3.1 --- Structure --- p.14 / Chapter 1.3.2 --- Function --- p.16 / Chapter 1.4 --- Follistatin (FS) --- p.17 / Chapter 1.4.1 --- Structure --- p.17 / Chapter 1.4.2 --- Function --- p.19 / Chapter 1.5 --- Temporal Variations in the GTH Expressional and Releasing Profile and Sex Steroid Level in the Goldfish --- p.19 / Chapter 1.5.1 --- Hormone changes during annual cycle --- p.20 / Chapter 1.5.2 --- Hormone changes during ovulatory cycle --- p.21 / Chapter 1.6 --- Objectives of the Present Study --- p.23 / Chapter Chapter 2 --- "Effects of Dopamine on the Expression of Gonadotropin (GTH) Subunits in the Dispersed Pituitary Cells of the Goldfish, Carassius auratus" --- p.26 / Chapter 2.1 --- Introduction --- p.26 / Chapter 2.2 --- Materials and Methods --- p.27 / Chapter 2.2.1 --- Materials --- p.27 / Chapter 2.2.2 --- Primary culture of dispersed goldfish pituitary cells --- p.28 / Chapter 2.2.3 --- mRNA analysis --- p.29 / Chapter 2.2.4 --- Data analysis --- p.30 / Chapter 2.3 --- Results --- p.30 / Chapter 2.3.1 --- Effects of DA on GTH-Iβ and GTH-IIβ expression --- p.30 / Chapter 2.3.2 --- Effects of DA D1 and D2 agonists on GTH-Iβ expression --- p.33 / Chapter 2.3.3 --- Effects of DA D1 and D2 antagonists on DA- inhibited GTH-Iβ expression --- p.33 / Chapter 2.3.4 --- Effects of α-adrenergic agonists on GTH-Iβ expression --- p.33 / Chapter 2.4 --- Discussion --- p.37 / Chapter Chapter 3 --- Seasonal Variation of Activin-regulated Goldfish Pituitary GTH-Ip and GTH-IIβ Expression and Evidence for the Involvement of Gonadal Steroids --- p.40 / Chapter 3.1 --- Introduction --- p.40 / Chapter 3.2 --- Materials and Methods --- p.42 / Chapter 3.2.1 --- Materials --- p.42 / Chapter 3.2.2 --- Gonadectomy of the goldfish --- p.42 / Chapter 3.2.3 --- Primary culture of dispersed pituitary cells --- p.43 / Chapter 3.2.4 --- mRNA analysis --- p.43 / Chapter 3.2.5 --- Data analysis --- p.44 / Chapter 3.3 --- Results --- p.44 / Chapter 3.3.1 --- Effects of goldfish activin B on the expression of GTH-Iβ and GTH-IIβ --- p.44 / Chapter 3.3.2 --- Seasonal variation of activin-regulated expression of GTH-Iβ and GTH-IIβ --- p.45 / Chapter 3.3.3 --- Effects of gonadectomy on basal and activin- regulated expression of GTH-Iβ and GTH-IIβ --- p.45 / Chapter 3.3.4 --- Effects of sex steroids on basal and activin- regulated expression of GTH-Iβ and GTH-IIβ in vitro --- p.50 / Chapter 3.4 --- Discussion --- p.57 / Chapter Chapter 4 --- Evidence for the Autocrine/Paracrine Regulation of Gonadotropin Expression by Activin in the Goldfish Pituitary --- p.61 / Chapter 4.1 --- Introduction --- p.61 / Chapter 4.2 --- Materials and Methods --- p.63 / Chapter 4.2.1 --- RNA isolation --- p.63 / Chapter 4.2.2 --- Reverse transcription-polymerase chain reaction (RT-PCR) --- p.63 / Chapter 4.2.3 --- Primary culture of goldfish pituitary cells --- p.63 / Chapter 4.2.4 --- Slot-blot analysis --- p.64 / Chapter 4.2.5 --- Data analysis --- p.64 / Chapter 4.3 --- Results --- p.65 / Chapter 4.3.1 --- Expression of activin βB subunit and activin type IEB receptor in the goldfish pituitary --- p.65 / Chapter 4.3.2 --- Effects of human activin A on goldfish GTH-Iβ and GTH-IIβ expression --- p.65 / Chapter 4.3.3 --- Effects of follistatin on basal and activin- regulated GTH-Iβ and GTH-IIβ expression --- p.69 / Chapter 4.4 --- Discussion --- p.69 / Chapter Chapter 5 --- General Discussion --- p.77 / Chapter 5.1 --- Overview --- p.77 / Chapter 5.2 --- Contribution of the Present Study --- p.78 / Chapter 5.2.1 --- Dopamine as a potential neuroendocrine regulator in the differential regulation of GTH-Iβ and GTH-IIβ expression --- p.78 / Chapter 5.2.2 --- Seasonal variation of the effects of activin on GTH-Iβ and GTH-IIβ expression --- p.79 / Chapter 5.2.3 --- Autocrine/paracrine regulation of GTH expression by activin --- p.79 / Chapter 5.3 --- Future Prospects --- p.81 / References --- p.84

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323450
Date January 2001
ContributorsYuen, Chi Wai., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xvii, 106 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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