Adeno-associated virus type 2 (AAV-2) integrates preferentially into a defined site on human chromosome 19, and has been developed as a gene therapy vector. We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana via Agrobacterium-mediated transformation. PCR and sequence analysis confirmed the presence of rep78 in two plant lines. RT-PCR demonstrated rep78 transcription in one plant line, but protein could not be detected in either line. / Master of Science
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/9706 |
| Date | 24 September 2002 |
| Creators | Sisco, Daniel |
| Contributors | Biology, Lederman, Muriel L., Winkel, Brenda S. J., Rutherford, Charles L. |
| Publisher | Virginia Tech |
| Source Sets | Virginia Tech Theses and Dissertation |
| Detected Language | English |
| Type | Thesis |
| Format | ETD, application/pdf, application/pdf, application/pdf, application/pdf, application/pdf, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
| Relation | 03tableofcontents.pdf, 07references.pdf, 08resume.pdf, 04intro.pdf, 06resultsanddiscussion.pdf, 05materialsandmethods.pdf, 01title.pdf, 02abstract.pdf |
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