The aim of this work was to verify the hypothesis that eicosapentaenoic acid and docosahexaenoic acid favorably influence the inflammatory response in the organism through their influence on the expression of genes associated with the inflammatory process. The experiment was carried out on pigs fed with fish oil supplement and palm oil supplement. Certain experimental groups were additionally stimulated by lipopolysaccharide to induce a rapid onset of inflammatory response, with particular concern for the ability of polyunsaturated fatty acids n-3 to inhibit this process. I have been observing changes in gene expression for AdipoR1 and AdipoR2 adiponectin receptors, as well as expression in other genes related to the inflammatory process of LBP; ICAM1; IL-6; TNFalfa; IL-12. The determination of the expression of selected genes using qRT-PCR was used to process the results from the collected liver and adipose tissue samples. I statistically evaluated the expression results of the genes and displayed them as graphs. The favorable demonstrable effect of fish oil was only observed in the ADIPOR2 gene in the liver, in the non-stimulated lipopolysaccharide group (P <0.05). There was no detectable effect of fish oil on modulation of inflamation in pigs. However, we can deduce the causes of the failure from the experimental results, and in other studies we might be able to confirm the hypothesis. However, we can deduce the causes of the failure from the experimental results and we might be able to confirm the hypothesis in other studies.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:429989 |
| Date | January 2017 |
| Creators | Frólo, Michal |
| Source Sets | Czech ETDs |
| Language | Slovak |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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