Throughout adulthood, the brain continuously generates new neurons in two neurogenic regions: the subgranular zone of the hippocampus and the subventricular zone on the lateral wall of the lateral ventricles. These neurons have been shown to integrate into hippocampal and olfactory bulb circuitry, respectively. Nevertheless, their specific contribution to hippocampal or olfactory function remains unclear. Previous studies have tried to assess adult born neuron contribution to memory function by suppressing neurogenesis and examining the impact on memory acquisition. Although ablation of neurogenesis has been shown to impair performance in hippocampus dependent and olfactory tasks, many studies fail to see an effect. Compensation from residual cells in either system after ablation may underlie these contradictory findings. Thus, a more direct approach to answer this question would be to ablate adult born neurons after their incorporation into the memory trace. To do this, we established a double transgenic strategy to tag and selectively ablate adult born neurons with temporal control. Ablation of a population of predominantly mature, adult generated dentate granule cells did not prevent acquisition of contextual fear conditioning or Morris Water Maze memories. Removal of that same population of cells after training, however, led to memory degradation in three hippocampus dependent tasks. Similarly, post-training ablation of a population of adult generated olfactory interneurons
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impaired performance in an associative odour memory task, whereas pre-training ablation had no impact. Together, these data show that adult generated neurons form a crucial component of both hippocampal and olfactory memory traces.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/43375 |
| Date | 12 December 2013 |
| Creators | Arruda Carvalho, Maithe |
| Contributors | Frankland, Paul |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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