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Isolation and characterization of immunoglobulin G from Panthera leo in South Africa and Zimbabwe

While a decrease of wild felid population has led to disruption of conservation
programme, recent studies have shown the importance of immune regulation for
determining health outcomes and co-infection. Immunoglobulin G is important for
detecting and evaluating responses to infectious diseases and vaccination. But, there
is limited information on felid immunoglobulins and their role for functional immunity.
This study aimed at isolating and characterizing lion’s immunoglobulin G. Lions’ sera
(n = 68) were processed using the MagReSyn® magnetic beads and the final protein
concentration was determined using the Xpose™ Trinean Spectrophotometer. The
cross-reactivity of goat anti-cat immunoglobulin with sera of lions and other species
was analysed using ELISA. High cross-reactivity was observed in lions ranging from
87.7 to 100%, and low reactivity with rhino (22.4%) followed by chicken (0.01%). The
protein concentration from purified sera yielded 39.09 mg/ml. Molecular weight of lion
IgG 150-160 kDa was detected with both chains at 54-56 kDa and 24-26 kDa on SDS PAGE. These results indicate a potential aid in developing serological tools to monitor
exposure to micro-organisms of lions. / Agriculture and  Animal Health / M. Sc. (Agriculture)

Identiferoai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:unisa/oai:uir.unisa.ac.za:10500/27525
Date06 1900
CreatorsManamela, Tebogo Sabina
ContributorsKayoka, P. N., Morar-Leather, D.
Source SetsSouth African National ETD Portal
LanguageEnglish
Detected LanguageEnglish
TypeDissertation
Format1 online resource (68 leaves) : illustrations (some color), graphs (some color), color map, application/pdf

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