Agrobacterium tumefaciens is a gram-negative soil bacterium
that causes crown gall tumors on dicotyledenous plants. The
transferred DNA (T-DNA) portion of the A. tumefaciens tumor-inducing
(Ti) plasmid enters infected plant cells and integrates into
plant nuclear DNA. The T-DNA is accompanied into plant cells by the
VirD2 endonuclease covalently attached to its 5' end. VirE2, a
cooperative, single-stranded DNA-binding protein is also
transported into plant cells during infection by A. tumefaciens.
VirD2 and VirE2 contain nuclear localization signals (NLSs) and are
transported into the plant cell nucleus.
The location of functional domains by the insertion of Xhol linker oligonucleotides throughout virE2 is reported. A ssDNA binding domain was located in the C-terminal half of VirE2, as well two domains involved in cooperative single-stranded DNA binding. Further, we isolated a mutation in the central region of VirE2 that decreased tumorigenicity, but did not affect ssDNA binding. / Graduation date: 1997
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/34220 |
| Date | 18 July 1996 |
| Creators | Dombek, Priscilla Emily |
| Contributors | Ream, Walt |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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