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The Effect of Post-resistance Exercise Alcohol Ingestion on LPS-stimulated Cytokines

The purpose of this study was to examine the effect of post-resistance exercise alcohol ingestion on LPS-stimulated production of IFNγ, TNF-α, IL-1β, IL-6, IL-8, and IL-10. Recreationally resistance-trained men (n = 10, 25 ± 3 yr, 177 ± 7 cm, 83.8 ± 15.7 kg, 14.8 ± 8.5% body fat) and women (n = 8, 23 ± 2 yr, 161 ± 3 cm, 59.5 ± 6.0 kg, 26.5 ± 3.0% body fat) completed the study. Participants visited the laboratory for an initial visit at which time they were screened, familiarized with procedures, and had their 1-repetition maximum (1RM) back squat tested. Subsequently, participants visited the laboratory 2 more times and completed 2 identical heavy resistance exercise bouts (6 sets of 10 repetitions of 80% 1RM back squat) after which a beverage, either containing alcohol (alcohol condition, ALC; 1.09 g EtOH per kg fat free mass) or water (placebo condition, PLA), was administered. Blood samples were collected before exercise (PRE), and at 3 hours (3h) and 5 hours (5h) after exercise. Samples were stimulated with lipopolysaccharide (LPS) and cultured overnight. Supernatant was collected and analyzed for IFNγ, TNF-α, IL-1β, IL-6, IL-8, and IL-10. A significant (p < 0.05) main effect for time was found for IFNγ, TNF-α, and IL-1β (5h greater than PRE) and for IL-10 (5h less than PRE and 3h, 3h less than PRE). An interaction effect was found for IL-8 (ALC less than PLA at 5h) and for IL-6 (ALC greater than PLA at PRE and ALC less than PLA at 3h). For IL-6, ALC was less at 3h than at PRE, and PLA was greater at 3h than at PRE. Overall, the LPS-stimulated cytokine response was pro-inflammatory by 5h. Alcohol consumed after heavy resistance exercise reduced LPS-stimulated production of IL-6 and IL-8 but not of IFNγ, TNF-α, IL-1β, or IL-10. These data indicate that alcohol affected inflammatory capacity but that the potential effect of alcohol on the production of cytokines in response to LPS was largely overwhelmed by the resistance exercise bout.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc804851
Date08 1900
CreatorsLevitt, Danielle E.
ContributorsVingren, Jakob L., McFarlin, Brian K., Hill, David Wilfred, 1952-
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formativ, 26 pages : illustrations, Text
RightsPublic, Levitt, Danielle E., Copyright, Copyright is held by the author, unless otherwise noted. All rights Reserved.

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