This project was designed to investigate the effects of carbon dioxide (CO2) levels on the
growth and pigment ratios (chemotaxonomy) of freshwater algal species typical to the
south Florida surface waters. Green algae, diatoms, and cyanobacteria were cultured
under 400 or 800 ppm CO3 in air for several weeks. Growth monitoring used a cell
counter, hemocytometer, and chlorophyll fluorescence. Pigments were analyzed using
HPLC-PDA. Experiments with certified CO2 concentrations (400, 600, 800, 1200 ppm)
were conducted with helium degassed ultrapure water and each of three culture media.
Theoretical and experimental pH values with water matched exactly. However, each
culture media proved to exhibit significant buffer capacity. Cell growth monitoring was
problematic except for the cyanobacterium Microcystis aeruginosa. That species
responded to increased CO2 (800 ppm) with increased growth rates as predicted. The
other species gave erratic results mainly due to difficulties in obtaining valid consistent
cell counts. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2018. / FAU Electronic Theses and Dissertations Collection
| Identifer | oai:union.ndltd.org:fau.edu/oai:fau.digital.flvc.org:fau_40907 |
| Contributors | Bermudez, Jeanne (author), Louda, J. W. (Thesis advisor), Florida Atlantic University (Degree grantor), Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry |
| Publisher | Florida Atlantic University |
| Source Sets | Florida Atlantic University |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation, Text |
| Format | 83 p., application/pdf |
| Rights | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder., http://rightsstatements.org/vocab/InC/1.0/ |
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