We compared a kinetic method (KM) and a colorimetric method (CM) for
measuring erythrocyte aspartate aminotransferase (EAST) activity. Twenty-three
healthy college students including 7 men and 16 women, aged from 22 to 40 years,
participated in this study. Vitamin B-6 status was assessed by EAST activity
coefficient (EAST-AC), the ratio of EAST stimulated activity by adding PLP in vitro
(EAST-SA) to basal activity (EAST-BA). These subjects' EAST indices (EAST-BA,
EAST-SA and EAST-AC) were compared to their plasma PLP concentration and their
dietary intake of vitamin B-6 as determined by the food frequency questionnaire (FFQ)
and 3-day dietary record.
There was a significant correlation (r=0.59, p<0.01) in EAST-BA obtained by
the two methods, while the correlation of EAST-SA values between the two methods
was not significant (r=0.40, p=0.06). EAST-AC obtained with KM was linearly
associated (r=0.57, p<0.01) to EAST-AC obtained with CM, but was 1.26 times higher (p<0.01) than that with CM. Thus, the method used for the determination of the
normal EAST-AC value needs to be noted. None of EAST indices measured were
significantly correlated with plasma PLP concentration.
There was a high correlation for vitamin B-6 intake (r=0.65, p<0.01) and the
ratio of vitamin B-6 to dietary protein (r=0.58, p<0.01) estimated between the FFQ
and the 3-day dietary record. The results suggested the high validity of the FFQ for
determining vitamin B-6 intake. Neither of these dietary methods was, however,
correlated with any EAST activity indices or the plasma PLP concentration. / Graduation date: 1995
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/27490 |
| Date | 06 March 1995 |
| Creators | Iwakiri, Yasuko |
| Contributors | Miller, Lorraine T. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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