BACKGROUND: Cells, growth factors (GFs), and scaffold are three essential factors for tissue engineering. Our previous studies suggested that multiple applications of human amnion growth factors (AGF) into osseous defects could “mimic in-utero” growth. However, micro-gaps still exist between the scaffold and recipient tissue. We hypothesized that hyaluronic acid (HA) could act an accessory scaffold and gradually release active components of AGF and improve bone healing.
MATERIALS AND METHODS: Calvaria from 50 7–9-day old CD1 neonatal mice were harvested, and a 2 mm defect punch made in each one. A type I collagen membrane with AGF alone or with HA at different concentrations applied over the defect. The culture medium was changed every 2-3 days and collected for alkaline phosphatase (ALP) and protein analysis.
RESULTS: A single dose of AGF combined with 0.125% HA increased cellular infiltration into the defect area more than AGF with no HA or a lower concentration of HA (0.0625%). A single dose of AGF with HA can improve bone healing.
CONCLUSION: A single dose of AGF with HA as an extra scaffold and a carrier can achieve bone formation like multiple dosages of AGF and reduce the number of clinical applications needed.
| Identifer | oai:union.ndltd.org:bu.edu/oai:open.bu.edu:2144/42686 |
| Date | 13 June 2021 |
| Creators | Alibhai, Karishma |
| Contributors | Dibart, Serge |
| Source Sets | Boston University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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