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Previous issue date: 2013-06-19 / The main specie of marine shrimp raised at Brazil and in the world is Litopenaeus vannamei,
which had arrived in Brazil in the `80s. However, the entry of infectious myonecrosis virus
(IMNV), causing the infectious myonecrosis disease in marine shrimps, brought economic
losses to the national shrimp farming, with up to 70% of mortality in the shrimp production.
In this way, the objective was to evaluate the survival of shrimps Litopenaeus vannamei
infected with IMNV using the non parametric estimator of Kaplan-Meier and a model of
frailty for grouped data. It were conducted three tests of viral challenges lasting 20 days each,
at different periods of the year, keeping the parameters of pH, temperature, oxygen and
ammonia monitored daily. It was evaluated 60 full-sib families of L. vannamei infected by
IMNV in each viral challenge. The confirmation of the infection by IMNV was performed
using the technique of PCR in real time through Sybr Green dye. Using the Kaplan-Meier
estimator it was possible to detect significant differences (p <0.0001) between the survival
curves of families and tanks and also in the joint analysis between viral challenges. It were
estimated in each challenge, genetic parameters such as genetic value of family, it`s respective
rate risk (frailty), and heritability in the logarithmic scale through the frailty model for
grouped data. The heritability estimates were respectively 0.59; 0.36; and 0.59 in the viral
challenges 1; 2; and 3, and it was also possible to identify families that have lower and higher
rates of risk for the disease. These results can be used for selecting families more resistant to
the IMNV infection and to include characteristic of disease resistance in L. vannamei into the
genetic improvement programs / A principal esp?cie de camar?o marinho cultivado no Brasil e no mundo ? o Litopenaeus
vannamei, que teve entrada no Brasil nos anos 80. Contudo, a chegada do v?rus da mionecrose
infecciosa (IMNV), causador da doen?a da mionecrose infecciosa em camar?es marinhos,
trouxe preju?zos econ?micos ? carcinicultura nacional, com mortalidades de at? 70% na
produ??o de camar?es. Dessa maneira, objetivou-se avaliar a sobreviv?ncia de camar?es
Litopenaeus vannamei infectados pelo v?rus da mionecrose infecciosa utilizando o estimador
n?o param?trico de Kaplan-Meier e um modelo de fragilidade para dados grupados. Foram
conduzidos tr?s testes de desafios virais com dura??o de 20 dias cada, em diferentes ?pocas
do ano, mantendo-se os par?metros de pH, temperatura, oxig?nio e am?nia controlados
diariamente. Foram avaliadas 60 fam?lias de irm?os completos de camar?es marinhos L.
vannamei infectadas pelo IMNV em cada desafio viral. Foi feita a confirma??o da infec??o
pelo IMNV atrav?s da t?cnica de PCR em tempo real utilizando o corante Sybr Green.
Atrav?s do Estimador de Kaplan-Meier foi poss?vel detectar diferen?as significativas
(p<0,0001) entre as curvas de sobreviv?ncia das fam?lias, entre as curvas de sobreviv?ncia
entre os tanques por desafio viral e tamb?m na an?lise conjunta dos desafios virais. Foram
estimados, em cada desafio, par?metros gen?ticos como o valor gen?tico de fam?lia e sua
respectiva taxa de risco (fragilidade), e a herdabilidade na escala logar?tmica por meio do
modelo de fragilidade para dados grupados. A herdabilidade estimada foi respectivamente
0,59; 0,36; e 0,59 nos desafios virais 1; 2; e 3; e tamb?m foi poss?vel identificar as fam?lias
que possuem as menores e maiores taxas de risco para a doen?a. Esses resultados podem ser
utilizados para sele??o de fam?lias mais resistentes ? infec??o pelo IMNV e a inclus?o da
caracter?stica de resist?ncia ? doen?a em programas de melhoramento gen?tico de L.
vannamei
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17184 |
| Date | 19 June 2013 |
| Creators | Kurkjian, Karin |
| Contributors | CPF:92257577604, http://lattes.cnpq.br/5958935216683927, Borba, Luis Henrique Fernandes, CPF:09983700883, http://lattes.cnpq.br/9170284877246032, L?cio, Paulo S?rgio Marinho, CPF:49188178404, Braz, Regina de F?tima dos Santos, CPF:00567556867, http://lattes.cnpq.br/9442000157600332, Cunha, Eliz?ngela Em?dio |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Produ??o Animal, UFRN, BR, Sistemas de Produ??o Sustent?veis no Semi-?rido; Caracteriza??o, conserva??o e melhoramento gen?tico |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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