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Characterisation of a tannin acylhydrolase from a ruminal selenomonad / by Ian Skene.

Bibliography: leaves 189-205. / xi, 205 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The aim of this PhD project is to screen feral goat rumen fluid for the presence of new organisms that may play a role in the detoxification of tannins and to investigate their mechanisms of action. An enrichment experiment is conducted to screen rumen fluid for anaerobic bacteria capable of growing in the presence of high levels of "Acacia" condensed tannin. Four morphologically-distinct bacteria are isolated, confirming that resistance is a property shared by more than one organism. One isolate is chosen at random for further characterisation and is identified as a strain of "Selenomonas ruminantium" subspecies "ruminantium". It is arbitrarily designated strain K2. "Selenomonas ruminantium" K2 is shown to be not only tannin-resistant but also able to grow on tannic acid. It is proposed that this bacterium obtained energy for growth from tannic acid. The thesis examines the molecular mechanisms controlling tannin resistance or tannin degradation in rumen microorganisms. / Thesis (Ph.D.)--University of Adelaide, Dept. of Animal Science, 1997

Identiferoai:union.ndltd.org:ADTP/279874
Date January 1996
CreatorsSkene, Ian
Source SetsAustraliasian Digital Theses Program
Languageen_US
Detected LanguageEnglish

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