Joie Jie Wang. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 121-132). / ABSTRACT --- p.i / ACKNOWLEDGEMENTS --- p.iii / LIST OF TABLE --- p.vii / LIST OF FIGURES --- p.viii / LIST OF ABBREVIATIONS --- p.xii / Chapter CHAPTER I. --- INTRODUCTION --- p.1 / Chapter I.1. --- Fibre tracts of the rat spinal cord --- p.1 / Chapter I.2. --- Histopathological responses to spinal cord injuries --- p.2 / Chapter I.3. --- Failure of CNS regeneration --- p.4 / Intrinsic inability of CNS neurons themselves to regenerate --- p.4 / Inappropriate synapse without normal functioning --- p.5 / Progressive necrosis and cystic cavities --- p.5 / Autoimmune explanation for the failure of regeneration --- p.6 / Glial scarring --- p.6 / Absence of Schwann cells in the CNS --- p.7 / Lack of requisite growth factors --- p.8 / Chapter I.4. --- The use of transplants --- p.9 / Transplants of fetal nerve tissues --- p.9 / Transplants of peripheral nerve tissues --- p.10 / Transplants of neuroglial cells --- p.11 / Transplants of central neurons --- p.12 / Chapter I.5. --- Objectives of the present study --- p.13 / Chapter CHAPTER II. --- METERIALS AND METHODS --- p.15 / Chapter II.1. --- Hemisection of rats --- p.15 / Chapter II.2. --- Preparation of purified cortical astrocytes --- p.15 / Chapter II.3. --- Scanning electron microscopy (SEM) --- p.18 / Chapter II.4. --- HistologýؤLight microscopy --- p.19 / Chapter II.5. --- Measurement of volume of scar tissue --- p.19 / Chapter II.6. --- Immunofluorescence staining --- p.20 / Chapter II.7. --- Transmission electron microscopy --- p.23 / Chapter II.8. --- Comparison of expression of various proteins in the spinal cord --- p.24 / Polyacrylainide gel electrophoresis --- p.24 / Western blotting --- p.26 / Chapter CHAPTER III. --- RESULTS --- p.28 / Chapter III.1. --- Survival of cultured astrocytes --- p.28 / Chapter III.2. --- Light microscopy --- p.28 / Hemotoxylin and Eosin staining --- p.28 / Toluidine Blue staining --- p.30 / PHAL labelled astrocytes --- p.31 / Immunofluorescence staining --- p.32 / N-CAM --- p.32 / GFAP --- p.33 / NF --- p.34 / Chapter III.3. --- Transmission electron microscopy (TEM) --- p.35 / Chapter III.4. --- Determination of the volume of scar tissue --- p.37 / Chapter III.5. --- Gel electrophoresis --- p.38 / Chapter III.6. --- Immunoblotting and densitometry --- p.38 / Chapter CHAPTER IV. --- DISCUSSION AND CONCLUSIONS --- p.115 / REFERENCES --- p.121
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_319176 |
| Date | January 1993 |
| Contributors | Wang, Joie Jie., Chinese University of Hong Kong Graduate School. Division of Basic Medical Sciences. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiii, 132 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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