Adoptive transfer has been an important tool for the study of immune responses in mice. In vivo demonstrations and in some cases the discovery of the nature and role of cell populations and subpopulations in the immune responses of this animal have often depended on the use of some version of adoptive transfer. Because lines of syngeneic animals are essential for the systematic use of adoptive transfer techniques, mice and rats are the only animal models in which this system has been used. Genetic barriers prevent its use in the study of human immunology and, until recently, in studies of the immunology of animals of agricultural interest. The advent of cloning has made it possible to derive cloned lines of cattle (Cibelli et al., 1998). As members of the same clone demonstrate complete histocompatibility, it should be possible to transfer cells from immunized to unimmunized members of the same clone. During the course of this study we were able to show that by means of adoptive transfer it was possible to confer the ability to mount secondary responses upon primary immunization from an immunized donor to another member of the clone via the blood transfusion. Furthermore, we were able to adoptively transfer increased immune responsiveness from immunologically mature primed adult members of the clone to young immunologically immature recipients. All these data point to the fact that after primary immunization all recipients generated an antigen-specific antibody response with the characteristics of a secondary immune response, indicating the active functioning of memory cell pools transferred from the donors. We showed that antigen-specific polyclonal responses could be transferred by means of a single blood transfusion, which indicated that using blood transfusion it is possible to transfer multiple clones of antigen-specific B cells. Finally, by use of a medically relevant antigen for donor immunization, we were able to demonstrate the suitability of this technique for the adoptive transfer of antibody responses of biomedical and biodefense interest. We were able to show the kinetics of antibody generation and class switching as well as polyclonality of the humoral response formed in recipients. These results demonstrated that the advent of cloning has brought to experimental bovine immunology access to the investigational power of adoptive transfer and other approaches that require the transfer of cell populations from manipulated donors to recipients. I believe that the use of adoptive transfer for the study of B-cell sponsored responses will invite the extension of this approach to in vivo study of areas such as bovine regulatory T cells and other cell dependent phenomena that are particularly well-studied by adoptive transfer.
| Identifer | oai:union.ndltd.org:UMASS/oai:scholarworks.umass.edu:dissertations-3957 |
| Date | 01 January 2004 |
| Creators | Davidyuk, Galina |
| Publisher | ScholarWorks@UMass Amherst |
| Source Sets | University of Massachusetts, Amherst |
| Language | English |
| Detected Language | English |
| Type | text |
| Source | Doctoral Dissertations Available from Proquest |
Page generated in 0.0026 seconds