Four lipogenic enzymes were measured in liver and adipose tissues of 2-, 6-, 18- and 27-month-old male Fischer 344 rats. Adipose ATP-citrate lyase (EC 4.1.3.8; ATP-CL) activity increased 1.8-fold between 2 and 6 months of age. Hepatic ATP-CL activity underwent an overall 84% reduction between 2 and 27 months of age with maximum activity at 18 months. Adipose tissue fatty acid synthase (FAS) activity exhibited a linear 54% reduction between 2 and 27 months of age; while hepatic FAS activity declined, biphasically, to 14.2 IU/g protein by 27 months. Opposing age-related patterns of glucose-6-phosphate dehydrogenase (EC 1.1.1.49; G6PD) activity were observed in adipose tissue and liver. Adipose activity decreased between 2 and 6 months to 132.9 IU/g protein, and was unchanged thereafter; whereas liver G6PD activity increased to a maximum of 149.0 IU/g protein at 27 months. Malic enzyme (EC 1.1.1.40; ME) activity decreased between 2 and 6 months of age in both tissues. These data suggest diminished lipogenic capacity with age.
| Identifer | oai:union.ndltd.org:arizona.edu/oai:arizona.openrepository.com:10150/291486 |
| Date | January 1990 |
| Creators | Kroening, Cheryl Ann, 1963- |
| Contributors | Reid, Bobby L. |
| Publisher | The University of Arizona. |
| Source Sets | University of Arizona |
| Language | en_US |
| Detected Language | English |
| Type | text, Thesis-Reproduction (electronic) |
| Rights | Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. |
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