The objective of this study is to demonstrate differences between erythroid and nonerythroid iron metabolism. Erythroid specific gene expression for transferrin receptor (TfR) which is a transporter of transferrin-bound iron, and for ferrochelatase which uses internalized iron to form heme, is found in dimethylsulfoxide-inducible murine erythroleukemia (MEL) cells. During MEL cell differentiation which parallels erythroid differentiation in vivo, TfR mRNA increases resulting from transcriptional activation of the gene and increased mRNA stability while ferrochelatase mRNA level increases with a 2.7 kb mRNA being induced preferentially. Such preferential induction is effected by utilization of an upstream polyadenylation signal. / TfR-deficient Chinese hamster ovary cells were used to study nonerythroid iron metabolism. Our results show these cells need iron for growth and DNA synthesis and acquire that from transferrin and FeSO$ sb4$ in the medium by a TfR-independent mechanism.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.39397 |
| Date | January 1992 |
| Creators | Chan, Roxanne Yuen Yee |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001306350, proquestno: NN80339, Theses scanned by UMI/ProQuest. |
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