Due to a 195 fold cardiac overexpression of beta2-Adrenoreceptor (beta2AR), the hearts of transgenic TG4 mice are chronically overstimulated and, indeed, show little stimulatory response to further betaAR agonists. Previous investigations had suggested that signaling from the overexpressed beta 2ARs was dampened in the atria of TG4 mice. Regulators of G-protein Signaling (RGSs) are a family of negative regulators of G-Protein Coupled Receptor (GPCR) signaling that are frequently induced by GPCR stimulation. Using an RT-PCR based strategy, we have identified RGS5 as being a candidate RGS that is up-regulated in the atria of TG4 mice. Northern blot analysis demonstrated that RGS5 levels are 2--3 fold higher in the atria of TG4 mice when compared to their non-transgenic littermates. To further characterize RGS5 expression, we generated an RGS5 specific anti-serum. Results indicate that RGS5 is a housekeeping RGS in the heart and in skeletal muscle but its betaAR mediated induction in the atria suggests that it also has a highly specialized function.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.80318 |
| Date | January 2003 |
| Creators | Li, Xiao Yu, 1973- |
| Contributors | Greenwood, Michael T. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Anatomy and Cell Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002031531, proquestno: AAIMQ98687, Theses scanned by UMI/ProQuest. |
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