Return to search

Effects of an interferon inducer, pI:C, on the graft-versus-host reaction

Polyinosinic: polycytidylic acid (pI:C), an interferon (IFN) inducer, was used to investigate the role of IFN in the graft-versus-host reaction (GVHR), induced by injecting parental cells into F1 recipients and assessed for immunosuppression and pathological lesions. / PI:C-treatment of recipients, but not donors, before GVHR-induction suppressed the GVHR, an effect seen only with C57BL/6 (B6) and not A donor cells. Using fluorescein-labelled donor cells, pI:C-treatment was seen to cause a marked decrease in donor cell survival after 2 days. Elimination of donor cells was specific for the B6 donor, was associated with increased natural killer (NK) cell but not macrophage cytotoxic activity, was radioresistant and anti-asialo GM1 sensitive, evidence supporting NK-mediated rejection. / Plotting donor cell recovery against the number of cells injected into variously treated recipients indicated that in unstimulated mice a constant proportion of the injected cells were rejected, pI:C increasing that proportion, suggesting that pI:C changes F1 NK target repertoire. / PI:C-treatment after GVHR-induction increased the severity of the GVHR, especially soon after GVHR-induction, the effect waning afterwards. No strain dependence was observed. / These results demonstrate that IFN/IFN-activated cells play an important role in the regulation and in the immunosuppression/pathogenesis of a GVHR.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.74294
Date January 1989
CreatorsPeres, Amos
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageDoctor of Philosophy (Department of Physiology.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 001232613, proquestno: AAINN63531, Theses scanned by UMI/ProQuest.

Page generated in 0.0024 seconds