Three known mammalian endothelins, ET-1, ET-2, and ET-3, are each encoded by separate genes and expressed in a variety of vascular and nonvascular tissues. Two subtypes of endothelin receptors have been identified and termed endothelin-A and endothelin-B receptors (ET-A and ET-B). In the first part of this study, the ET-A gene was disrupted in mouse embryonic stem cells to generate mice deficient in ET-A. / In the second part of this study, we investigated a targeted disruption of the mouse ET-B gene that results in aganglionic megacolon associated with coat color spotting, resembling a hereditary syndrome of mice, humans and other mammalian species (Waardenburg syndrome). / In the third part of this study, we demonstrated that a targeted disruption of the mouse endothelin-3 ligand (ET-3) gene produces a similar recessive phenotype of megacolon and coat color spotting. / Two types of endothelin converting enzymes (ECE-1 and ECE-2) have been recently identified. In the last part of this study, we investigated the expression of ECE-1 in human tissues using immunohistochemistry and in situ hybridization, and compared it to those of ET-1 and big ET-1. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.23382 |
| Date | January 1995 |
| Creators | Bayan, Farideh |
| Contributors | Giaid, A. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Pathology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001481324, proquestno: MM12158, Theses scanned by UMI/ProQuest. |
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