Acute administration of estrogen results in the vasodilatation and in the release of nitric oxide (NO) that occurs through activation of the serine-threonine kinase Akt/protein-kinase-B (PKB), which is known to increase the eNOS activity. 10-8 M of 17-beta-estradiol resulted in a left shift of the vasodilatory response to Ach in preconstricted aortic rings from oophorectomized rats (EC50 = 0.7 x 10-8 M with 17-beta-estradiol and 0.15 x 10-7 M of Ach without 17-beta-estradiol, P < 0.05). The effect was blocked by pre-treatment with Wortmannin, a PI(3)K inhibitor. AKT/PKB was phosphorylated in endothelial cells (EC) as early as 1-minute after estradiol-stimulation. Phosphorylation of eNOS and NO release in EC treated with 17-beta-estradiol were also increased. We conclude that the AKT/PKB pathway is involved in the acute release of NO by estrogen.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.33757 |
| Date | January 2001 |
| Creators | Flórián, Mária, 1953- |
| Contributors | Magder, Sheldon (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Physiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001862509, proquestno: MQ78874, Theses scanned by UMI/ProQuest. |
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