The objective of the first experiment was to determine the effects of storage at 22aC vs. 4aC on the motility and percentage of membrane-intact sperm (%MIS) of epididymal mouse sperm. Testicles were allocated into 22aC or 4aC treatment groups and stored for 24 or 48 hours. Additional testicles were allocated into the 4aC treatment for storage for 72 or 96 hours. Sperm was collected and analyzed at each time point. Storage at 22aC lowered motility and %MIS (P<0.05) when compared with control sperm and 4aC sperm at both the 24 and 48 hours. Motility and %MIS of 4aC sperm did not decrease when compared with the control until after 72 hours of storage. The second experiment evaluated the effects of 22aC vs. 4aC storage for 24 and 48 hours on epididymal dog sperm. Motility and %MIS of the 22aC sperm was lower than that of the 4aC sperm and the control (P<0.05) at 24 and 48 hours. Motility of the 4aC sperm was lower than the control at 24 and 48 hours (P<0.05), however %MIS was not lower than the control until 48 hours. The third experiment tested the effect of cryopreservation on epididymal dog sperm. Sperm was frozen immediately (A), after 48 hours at 4aC in liquid (B) or after 48 hours at 4aC of the whole testicle (C). Both pre-freeze (PF) and post-thaw (PT) motility and %MIS of B and C were lower than A (P<0.05). PT values were lower than PF values in all treatments (P<0.05). PT motility of B was lower than C (0 vs. 35.0¡Ó3.6%). Storage at 4aC allows collection of motile epididymal mouse and dog sperm for several days after death. Dog testicles can be refrigerated for 2 days and epididymal sperm frozen with PT motility and %MIS of 35 and 62%.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-1115102-091515 |
| Date | 15 November 2002 |
| Creators | Stilley, Karla |
| Contributors | Robert Godke, Dale Paccamonti, Earle Pope |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-1115102-091515/ |
| Rights | unrestricted, I hereby grant to LSU or its agents the right to archive and to make available my thesis or dissertation in whole or in part in the University Libraries in all forms of media, now or hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertation. |
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